While the study of the phylum Tardigrada has increased greatly in recent years, this research has been hampered by difficulties in obtaining reliable DNA sequence. For example, some researchers still rely solely on morphological identifications to proclaim new species, when DNA sequence would tell a different story. This poster will describe new oligonucleotides, thermal cycler parameters, DNA extraction techniques, and PCR cleanup steps designed to deal with this problem for sequencing single specimens. Specifically, three regions of DNA will be addressed; namely, the internal transcribed spacer and the 18s part of the small subunit, both of the rDNA, as well as the cytochrome oxidase subunit I of the mtDNA. This poster will address techniques allowing researchers to get sequence from all three DNA regions from the same animal, which presents a unique combination ideal for DNA barcoding, population genetics, phylogenetics, and species delimitation. These types of analyses are perfect for comparing tardigrades across multiple LTER sites and within the same site. Brigham Young University, Baker University, and Fresno City College are currently working on processing samples from all the North American LTER sites to analyze the tardigrade biodiversity. This information provides the perfect opportunity to further this goal, and any future tardigrade work at any LTER site will be able to benefit as well.